Article ID Journal Published Year Pages File Type
152938 Chemical Engineering Journal 2008 5 Pages PDF
Abstract

The effects of grafting poly(ethylene oxide) (PEO) onto the surface of a microchannel on the amplification efficiency of a microfabricated device for polymerase chain reaction (PCR) were studied. The PCR device was composed of a poly(dimethylsiloxane) microchannel and a glass-heating chip. The PEO chains were grafted using neat silane or Pluronic® F127, and the presence of PEO was confirmed by water contact angle analysis and X-ray photoelectron spectroscopy. The surface treatments with neat silane and 10% (w/v) Pluronic® F127 resulted in an increase in the PCR amplification of a 298-bp DNA product by 2.2-fold and 3.9-fold, respectively, while 1.7-fold and 2.3-fold increases, respectively, were observed for a 1.1-kb DNA product. Both treatments could effectively enhance PCR efficiency even when DNA template concentration was decreased from 20 to 2 pg/μl. Our results indicated that these simple surface treatments could therefore be used routinely to enhance the performance of similar devices.

Related Topics
Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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