| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 154224 | Chemical Engineering Journal | 2006 | 4 Pages |
Abstract
Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 °C and 200 rpm. Optimal incident light intensities depended on the initial cell densities.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Jiangtao Gao, Song Qin, Yicheng Zhang,