Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1942442 | Biochimica et Biophysica Acta (BBA) - Bioenergetics | 2012 | 10 Pages |
Biogenesis of the mitochondrial cytochrome c oxidase (COX) is a highly complex process involving subunits encoded both in the nuclear and the organellar genome; in addition, a large number of assembly factors participate in this process. The soil bacterium Paracoccus denitrificans is an interesting alternative model for the study of COX biogenesis events because the number of chaperones involved is restricted to an essential set acting in the metal centre formation of oxidase, and the high degree of sequence homology suggests the same basic mechanisms during early COX assembly. Over the last years, studies on the P. denitrificans Surf1 protein shed some light on this important assembly factor as a heme a binding protein associated with Leigh syndrome in humans. Here, we summarise our current knowledge about Surf1 and its role in heme a incorporation events during bacterial COX biogenesis. This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes.
► Paracoccus denitrificans is a suitable model for COX biogenesis. ► Surf1 abstracts heme a from its site of synthesis, heme a synthase. ► Surf1 provides a protein-bound heme a pool for COX biogenesis. ► Surf1 facilitates heme a incorporation into COX subunit I.