Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1971876 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology | 2016 | 7 Pages |
Abstract
We have previously demonstrated that melatonin (MEL) blocks the inhibition of the intestinal Ca2Â + absorption caused by menadione (MEN). The purpose of this study were to determine whether MEL not only restores but also prevents the intestinal Ca2Â + absorption inhibited either by MEN or BSO, two drugs that deplete glutathione (GSH) in different ways, and to analyze the mechanisms by which MEN and MEL alter the movement of Ca2Â + across the duodenum. To know this, chicks were divided into four groups: 1) controls, 2) MEN treated, 3) MEL treated, and 4) treated sequentially with MEN and MEL or with MEN and MEL at the same time. In a set of experiments, chicks treated with BSO or sequentially with BSO and MEL or with BSO and MEL at the same time were used. MEL not only restored but also prevented the inhibition of the chick intestinal Ca2Â + absorption produced by either MEN or BSO. MEN altered the protein expression of molecules involved in the transcellular as well as in the paracellular pathway of the intestinal Ca2Â + absorption. MEL restored partially both pathways through normalization of the O2â levels. The nitrergic system was not altered by any treatment. In conclusion, MEL prevents or restores the inhibition of the intestinal Ca2Â + absorption caused by different GSH depleting drugs. It might become one drug for the treatment of intestinal Ca2Â + absorption under oxidant conditions having the advantage of low or null side effects.
Keywords
DABplasma membrane Ca2 +-ATPasePMCA1bcalbindin D9kγ-GCSNCX1Na+/Ca2 + exchangerMELBSOiNOSNBTGSSGGAPDHGSH3,3′-diaminobenzidineclaudinsI/RO2−superoxide anion superoxide aniontight junctionsischemia and reperfusionnitro blue tetrazoliumOxidative stressGastrointestinalinducible nitric oxide synthaseSaline solutionMenMelatoninmenadioneNitric oxideCalbindin D28KGlutathioneoxidized glutathioneglyceraldehyde-3-phosphate dehydrogenase
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Authors
Vanessa Areco, Valeria Rodriguez, Ana Marchionatti, Agata Carpentieri, Nori Tolosa de Talamoni,