Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1972757 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology | 2011 | 10 Pages |
Abstract
Apolipophorin-III (apoLp-III), a hemolymph protein that facilitates lipid transport in aqueous media in insects was recently shown to play a role in insect immune activation. Here, we report another novel possible function of apoLp-III in insects. To identify genes affected by apoLp-III expression in larvae, we decreased endogenous apoLp-III mRNA in Hyphantria cunea (Hc) through RNA interference; subsequently, we observed lower levels of antioxidant enzymes, including manganese superoxide dismutase (MnSOD), glutathione S-transferase, and immune proteins. Knockdown of Hc apoLp-III led to decreased MnSOD expression in fat body tissues and elevated superoxide anion levels in Hc fat body cells, suggesting that Hc apoLp-III is involved in the action and/or expression of antioxidant enzymes, especially MnSOD.
Keywords
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Authors
Yong Il Kim, Hong Ja Kim, Yong Min Kwon, Young Jin Kang, In Hee Lee, Byung Rae Jin, Yeon Soo Han, Iksoo Kim, Hyang Mi Cheon, Nam Gyu Ha, Sook Jae Seo,