Identification of Plasmalogen in the gut of silkworm (Bombyx mori)

Herbivorous insect species are constantly challenged with endogenous and exogenous oxidative stress. Consequently, they possess an array of antioxidant enzymes and small molecular weight antioxidants. Lipid-soluble small molecular antioxidants, such as tocopherols, have not been well studied in insects but may play important antioxidant roles. In this study, we identified plasmalogen phosphatidylethanolamines (pPEs) as well as α-, β/γ-, δ-tocopherol in the larvae of the silkworm Bombyx mori by LCMS analyses and examined their distribution. Plasmalogen are reported to inhibit the metal ion induced oxidation. The composition of tocopherols was the same among gut contents, gut tissues, and the other tissues. However, plasmalogens, a unique class of glycerophospholipids rich in polyunsaturated fatty acids and containing a vinyl ether bond at the sn-1 position, were mainly distributed in gut tissues. Plasmalogens might protect gut tissues from oxidation stress.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (82 K)Download as PowerPoint slideHighlights► Antioxidant, plasmalogen phosphatidylethanolamines and tocopherols were identified in the larvae of Bombyx mori by LCMS. ► The composition of tocopherols was the same among gut contents, gut tissues, and the other tissues. ► However, plasmalogen phosphatidylthanolamines were mainly distributed in gut tissues. ► Plasmalogens might protect gut tissues from oxidation stress.