Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1982290 | Insect Biochemistry and Molecular Biology | 2011 | 6 Pages |
Recent advances in mass spectrometry (MS) technology have facilitated the detection and quantification of minor components in organisms and the environment. In this study, we successfully identified 20-hydroxyecdysone (20E) in first instar nymphs (7 days after hatching) of the scorpion Liocheles australasiae, using tandem mass spectrometry combined with high-performance liquid chromatography (LC/MS/MS). This substance was not found in adults after the fifth stage. Other possible molting hormone candidates such as makisterone A (MaA) and ponasterone A (PoA), both of which are reported to be the molting hormones of a few arthropod species, were not detected in this scorpion. The ligand–receptor binding of 20E and its analogs was quantitatively evaluated against the in vitro-translated molting hormone receptor, the heterodimer of ecdysone receptor (EcR) and the retinoid X receptor (RXR) of L. australasiae (LaEcR/LaRXR). The concentrations of ecdysone (E), MaA, 20E, and PoA that are required to inhibit 50% of [3H]PoA binding to the LaEcR/LaRXR complex were determined to be 1.9, 0.69, 0.05, and 0.017 μM, respectively. The activity profiles of these 4 ecdysteroids are consistent with those obtained for the molting hormone receptors of several insects. The binding of a non-steroidal E agonist, tebufenozide, to EcR was not observed even at high concentrations, indicating that the structure of the ligand-binding pocket of LaEcR is not favorable for interaction with tebufenozide.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (235 K)Download as PowerPoint slideHighlights► 20-Hydroxyecdysone was detected in the 1st instar nymphs of scorpions Liocheles australasiae. ► Binding affinity of ecdysteroids to the in vitro-translated EcR/RXR were measured quantitatively. ► Non-steroidal ecdsyone agonist, tebufenozide, did not bind to the in vitro-translated EcR/RXR.