Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1982387 | Insect Biochemistry and Molecular Biology | 2011 | 8 Pages |
The winter moth (Operophtera brumata L., Lepidoptera: Geometridae) utilizes a single hydrocarbon, 1,Z3,Z6,Z9-nonadecatetraene, as its sex pheromone. We tested the hypothesis that a fatty acid precursor, Z11,Z14,Z17,19-nonadecanoic acid, is biosynthesized from α-linolenic acid, through chain elongation by one 2-carbon unit, and subsequent methyl-terminus desaturation. Our results show that labeled α-linolenic acid is indeed incorporated into the pheromone component in vivo. A fatty-acyl-CoA desaturase gene that we found to be expressed in the abdominal epidermal tissue, the presumed site of biosynthesis for type II pheromones, was characterized and expressed heterologously in a yeast system. The transgenic yeast expressing this insect derived gene could convert Z11,Z14,Z17-eicosatrienoic acid into Z11,Z14,Z17,19-eicosatetraenoic acid. These results provide evidence that a terminal desaturation step is involved in the winter moth pheromone biosynthesis, prior to the decarboxylation.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (60 K)Download as PowerPoint slideHighlights► First methyl-terminus desaturase characterized, serving in moth mate pheromone communication. ► Terminal desaturase is highly expressed in abdominal tissue, suggesting the site of pheromone biosynthesis. ► In vivo labeling combined with in vitro heterologous expression presented the picture of winter moth pheromone biosynthesis pathway.