Altered juvenile hormone metabolism, reproduction and stress response in Drosophila adults with genetic ablation of the corpus allatum cells

Juvenile hormone (JH), which controls many developmental and physiological processes in Drosophila melanogaster, is synthesized de novo in the specialized endocrine glands, corpus allatum (CA). The present study concerns JH metabolism, reproduction and stress resistance in Drosophila with genetic ablation of a part of CA cells. The correlated regulation of JH biosynthesis and degradation in Drosophila adults has been found: ablation of CA cells led to (1) a dramatic decrease in activity of the key regulatory enzyme of JH biosynthesis, juvenile hormone acid methyl transferase and (2) a considerable increase in JH-hydrolyzing activity. It has been also shown that ablation of CA cells caused three significant physiological changes: (1) an increase in the intensity of response of JH degradation system to heat stress; (2) a disturbance of reproduction; (3) a decrease in stress resistance. Pharmacological rise of JH level rescued JH-hydrolyzing activity, fecundity and stress resistance in CA-ablated females. Pronouncedly, all the physiological effects caused by CA ablation were significant in females but not in males indicating a sexual dimorphism of JH physiological roles in Drosophila adults.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (110 K)Download as PowerPoint slideResearch highlights► Ablation of corpus allatum (CA) cells increases juvenile hormone (JH) degradation. ► Ablation of CA cells decreases juvenile hormone acid methyl transferase activity. ► Ablation of CA cells intensifies response of JH degradation system to heat stress. ► Ablation of CA cells results in a disturbance of reproduction in Drosophila females. ► Ablation of CA cells decreases stress resistance in Drosophila females.