| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2009320 | Pesticide Biochemistry and Physiology | 2014 | 9 Pages |
•Zoxamide decrease the expression of cytoskeleton-related protein of P. cactorum.•The up-regulated enzymes involved in sugar and detoxification metabolism protect P. cactorum against zoxamide.•Proteomics is useful in exploring the mode of action of fungicide and displaying the global response of protein in cells.
In this study, the global proteomic response of Phytophthora cactorum to zoxamide was evaluated using a two-dimensional gel electrophoresis (2-DE)-based proteomic approach. Among the 21 proteins identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS), four cytoskeleton-related proteins were down-regulated upon addition of zoxamide. Five detoxification metabolism enzymes, seven sugar metabolism proteins and one mitochondria-related protein were up-regulated by more than 2-fold in response to zoxamide. Taken together, these results suggest that zoxamide can decrease the expression of cytoskeleton-related proteins of P. cactorum, resulting in cell death; however, the up-regulation of detoxification metabolism-related enzymes may protect P. cactorum against zoxamide, and the up-regulation of proteins related to sugar metabolism and mitochondria may lead to the generation of more energy for detoxification metabolism. These data also suggest that proteomics may be useful not only in exploring the mode of action of fungicides but also for gaining insight into the resistance mechanisms that pathogens employ against fungicides.
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