| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2009414 | Pesticide Biochemistry and Physiology | 2012 | 5 Pages |
Two Descurainia Sophia populations, HB16 and HB08, were discovered in China and exhibit high resistance levels to tribenuron-methyl. Resistance ratio values of HB16 and HB08 were 153.47 and 651.20, respectively. The extractable acetohydroxy acid synthase (AHAS) activity was similar between resistant and susceptible populations. However, AHAS from HB16 and HB08 was less sensitive to inhibition of tribenuron-methyl comparing to susceptible population. The tribenuron-methyl I50 values for HB16 and HB08 were 46.25 and 54.07 greater than that for susceptible population respectively. Pro-197-Ser and Pro-197-Leu mutations were identified in AHAS extracted from HB16 and HB08 plants respectively. AHAS insensitivity of resistant D. Sophia caused by Pro197 mutation could be responsible for high resistance to tribenuron-methyl.
Graphical abstractThe resistance levels and resistance mechanisms of Descurainia Sophia (L.) to tribenuron-methyl were studied. The results confirmed that D. Sophia exhibited extremely high resistance levels to tribenuron-methyl in China and the AHAS sensitivity to tribenuron-methyl in vitro of resistant plants reduced significantly compared with AHAS from susceptible plants. Two different mutations at position of Pro197 were found in resistant plants of D. Sophia. Reduced sensitivity of AHAS caused by gene mutation at position of Pro197 may responsible for the high resistance levels to tribenuron-methyl.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Two flixweed (Descurainia Sophia L.) populations show high resistance levels to tribenuron-ethyl in China. ► The AHAS sensitivity to tribenuron-ethyl of two resistant populations reduced significantly. ► The AHAS genes of flixweed were sequenced. ► Proline at position 197 were substituted by leucine or serine.
