Mutation of the GABA receptor associated with fipronil resistance in the whitebacked planthopper, Sogatella furcifera

We found the A2′N mutation (index number for M2 membrane spanning region) in the GABA receptor subunit of fipronil-resistant Sogatella furcifera, by analyzing DNA sequences amplified from fipronil-resistant and -susceptible S. furcifera. In order to confirm the role of A2′N mutation in the fipronil resistance, we expressed the wild-type and A2′N mutant Drosophila GABA receptors in Drosophila Mel-2 cells stably. Amino acid sequences of three membrane spanning regions (M1-M3), which are important for binding of fipronil, are conserved between Drosophila and S. furcifera. So the results of A2′N mutant Drosophila GABA receptor suggest the role of A2′N mutation in fipronil-resistant S. furcifera. The membrane potential assay showed that the A2′N mutant Drosophila GABA receptor was not inhibited by fipronil at all, while the IC50 value of fipronil for wild-type Drosophila GABA receptor was 172 nM. These results suggest that A2′N mutation confers the resistance of fipronil in S. furcifera.