| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2009653 | Pesticide Biochemistry and Physiology | 2012 | 4 Pages |
The hemocyanin of Oncomelania hupensis (OhH) is essential for the survival of O. hupensis and may be an effective target for the development of new molluscicide. 4-(Chloroacetyl)catechol is a substrate analogue of OhH. In this study, we evaluated the toxicity of 4-(chloroacetyl)catechol to O. hupensis and Kunming mice. 4-(Chloroacetyl)catechol had strong molluscicidal activities and the molluscicidal activities was time and dose-dependent. With the increase of exposure time, the LC50 values of the 4-(chloroacetyl)catechol decreased from 6.5 mg/L (24 h) to 3.1 mg/L (72 h). The LC90 values decreased from 16.4 mg/L (24 h) to 4.9 mg/L (72 h). In the acute toxicity test of mice, no evident poisoning symptoms and no animal death were detected after 14 days’ continuous observation, which indicated that 4-(chloroacetyl)catechol was a low toxic substance for Kunming mice. These results indicated that 4-(chloroacetyl)catechol is potent molluscicides.
Graphical abstract4-(Chloroacetyl)catechol can inactivate oxygen-binding active site of hemocyanin from Oncomelania hupensis. 4-(Chloroacetyl)catechol had strong molluscicidal activities and the molluscicidal activities were time and dose-dependent.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Hemocyanin was isolated and purified from Oncomelania hupensis. ► 4-(Chloroacetyl)catechol can inactivate oxygen-binding active site of hemocyanin from Oncomelania hupensis. ► 4-(Chloroacetyl)catechol had strong molluscicidal activities and the molluscicidal activities were time and dose-dependent.
