Article ID Journal Published Year Pages File Type
2009699 Pesticide Biochemistry and Physiology 2010 5 Pages PDF
Abstract

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for cyhalofop-butyl was developed with a polyclonal antibody produced against a hapten (cyhalofop acid) conjugated with bovine serum albumin (BSA). The ELISA of cyhalofop-butyl showed an IC50 value of 0.067 ± 0.004 mg/l and the limit of detection (LOD, IC10) of 0.0029 ± 0.0001 mg/l at the optimal conditions. No significant cross-reaction to other structure-related compounds suggested high specificity for cyhalofop-butyl of the method. The average recoveries of cyhalofop-butyl from fortified water and soil were in the range of 83.2–119.7% and 80.1–104.0%, respectively. These data indicate that this method is a convenient analytical technique for monitoring cyhalofop-butyl in water and soil without purification steps.

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