| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2009733 | Pesticide Biochemistry and Physiology | 2012 | 8 Pages |
This paper compares the responses of conventional and transgenic soybean to glyphosate application in terms of the contents of 17 detectable soluble amino acids in leaves, analyzed by HPLC and fluorescence detection. Glutamate, histidine, asparagine, arginine + alanine, glycine + threonine and isoleucine increased in conventional soybean leaves when compared to transgenic soybean leaves, whereas for other amino acids, no significant differences were recorded. Univariate analysis allowed us to make an approximate differentiation between conventional and transgenic lines, observing the changes of some variables by glyphosate application. In addition, by means of the multivariate analysis, using principal components analysis (PCA), cluster analysis (CA) and linear discriminant analysis (LDA) it was possible to identify and discriminate different groups based on the soybean genetic origin.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A multivariate analysis allowed the classification of four soybean varieties. ► Glyphosate alters amino acid profile of conventional soybean. ► Glyphosate caused minor effects over amino acid profile of transgenic soybean. ► We present an alternative statistical tool for amino acid profile analysis.
