| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2009808 | Pesticide Biochemistry and Physiology | 2009 | 5 Pages |
Abstract
We investigated the inhibition of allene oxide synthase (AOS), a key enzyme in jasmonic acid biosynthesis, by miconazole. Kinetic analysis indicated that miconazole was a mixed-type inhibitor of AOS with a Ki value of approximately 8.4 ± 0.2 μM. Analysis of the interactions between miconazole and AOS by optical difference spectroscopy revealed that miconazole binding induces type II binding spectra with a Kd value of approximately 6.0 ± 0.2 μM.
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Authors
Keimei Oh, Yusuke Seki, Noboru Murofushi, Yuko Yoshizawa,