| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2009845 | Pesticide Biochemistry and Physiology | 2007 | 5 Pages |
This study was conducted to determine the in vitro effects of four commonly used pesticides (Nuarimol™, Fenarimol™, Parathion-methyl™ and 2,4-D™) on erythrocyte carbonic anhydrase (CA) activity from Saanen goats (SG). The enzyme was 262.57-fold purified by affinity chromatography and the purity was confirmed by SDS–PAGE. Inhibitory effect of the pesticides on the purified enzyme was determined using the CO2-hydratase activity method. IC50 values of the pesticides that caused inhibition were determined by means of activity percentage diagrams. The concentrations of Nuarimol™, Fenarimol™, and 2,4-D™ that inhibited 50% of the enzymatic activity were 0.352, 0.924 and 2.04 mM, respectively. Conversely, the enzyme activity was increased by parathion-methyl.
