Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2011796 | Pharmacological Reports | 2015 | 9 Pages |
Abstract
Over the last 40 years, there has been tremendous progress in understanding the biological reactions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is widely accepted that the generation of ROS and RNS is involved in physiological and pathophysiological processes. To understand the role of ROS and RNS in a variety of pathologies, the specific detection of ROS and RNS is fundamental. Unfortunately, the intracellular detection and quantitation of ROS and RNS remains a challenge. In this short review, we have focused on the mechanistic and quantitative aspects of their detection with the use of selected fluorogenic probes. The challenges, limitations and perspectives of these methods are discussed.
Keywords
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Authors
Karolina Debowska, Dawid Debski, Micael Hardy, Malgorzata Jakubowska, Balaraman Kalyanaraman, Andrzej Marcinek, RadosÅaw Michalski, Bartosz Michalowski, Olivier Ouari, Adam Sikora, Renata Smulik, Jacek Zielonka,