| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2011923 | Pharmacological Reports | 2012 | 8 Pages |
BackgroundThis study investigated the relationship between ethanol intake in rats and the resulting level of ethyl glucuronide (EtG) in rat hair.MethodsRats (n = 50) consumed a 10% ethanol solution for 4 weeks, then EtG was extracted from samples of their hair using a novel extraction procedure involving freezing and thawing. The EtG concentration was measured using gas chromatography and mass spectrometry. The animals voluntarily drank ethanol, with daily consumption in most rats exceeding 5 g/kg b.w. The silylated EtG was stable for at least 28 h. The limit of detection was 0.03 ng/mg, and the limit of quantification was 0.1 ng/mg.ResultsHair samples from rats that consumed ethanol had EtG levels ranging from 0.17–20.72 ng/mg in female rats and 0.15–13.72 ng/mg in males. There was a correlation between the amount of alcohol consumed and the EtG levels in hair from female (p < 0.01), but not male, rats.ConclusionThe method presented allows detection and quantification of EtG in rat hair. We also observed differences in EtG deposition in male and female rats.
