| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2011968 | Pharmacological Reports | 2011 | 5 Pages |
Abstract
This study investigated the presence of neuropeptide FF (NPFF) receptors on F-11 cells, a hybridoma derived from rat dorsal root ganglia (DRG) and mouse neuroblastoma. Binding experiments revealed a low density (4 fmol/mg) of high affinity (0.5 nM) [3H]-EYF binding sites in these cells. The whole-cell planar patch-clamp technique showed that dNPA, a selective NPFF2 agonist, increased the voltage-dependent potassium outward currents (about 30 pA/pF) by 21%; this reversible effect on sustained delayed potassium currents is blocked by tetraethylammonium. The similar effects of NPFF and opioid agonists on K+ currents in this cell line may explain their similar antinociceptive actions at the spinal level.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Catherine Mollereau, Michel Roumy, Jean-Marie Zajac,