Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2014747 | Plant Physiology and Biochemistry | 2016 | 12 Pages |
Abstract
Multiple stress response genes are controlled by transcription factors in a coordinated manner; therefore, these factors can be used for molecular plant breeding. CBF1/DREB1B, a known stress-inducible gene, was isolated from Arabidopsis thaliana and introduced into Salvia miltiorrhiza under the control of the CaMV35S or RD29A promoter. Under drought stress, relative water content, chlorophyll content, and the net photosynthetic rate were observed to be higher in the transgenic lines than in the wild type (WT). Moreover, O2â and H2O2 accumulation was observed to be lower in the transgenic lines. Additional analyses revealed that the AtDREB1B transgenic plants generally displayed lesser malondialdehyde (MDA) but higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities than the WT under drought stress. Quantitative real-time polymerase chain reaction of a subset of genes involved in photosynthesis, stress response, carbohydrate metabolism, and cell protection further verified that AtDREB1B could enhance tolerance to drought by activating different downstream DREB/CBF genes in the transgenic plants. Furthermore, no growth inhibition was detected in transgenic S. miltiorrhiza plants that expressed AtDREB1B driven by either the constitutive CaMV35S promoter or the stress-inducible RD29A promoter. Together, these results suggest that AtDREB1B is a good candidate gene for increasing drought tolerance in transgenic S. miltiorrhiza.
Keywords
PODRT-PCRLEAqRT-PCRPPTCATNBTRWCCaMVCBFSalvia miltiorrhizaABADREBO21DABMDAO2−OH−ROSnitroblue tetrazoliumHydrogen peroxideabscisic acidSingle oxygenDroughtdiaminobenzidineSuperoxide radicalsHydroxyl radicalsreverse transcription PCRLate embryogenesis abundant proteinsSODSuperoxide dismutaseAntioxidant defense systemC-repeat binding factorTranscription factorPhotosynthesisPhosphinothricinPhosphinothricin AcetyltransferasemalondialdehydeRelative water contentnet photosynthesis ratetranspiration rateStomatal conductanceH2O2quantitative real-time PCRfresh weightcauliflower mosaic viruspatPeroxidaseCatalaseReactive oxygen species
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Authors
Tao Wei, Kejun Deng, Yonghong Gao, Yu Liu, Meiling Yang, Lipeng Zhang, Xuelian Zheng, Chunguo Wang, Wenqin Song, Chengbin Chen, Yong Zhang,