Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2014868 | Plant Physiology and Biochemistry | 2013 | 8 Pages |
Abstract
Potato vacuolar acid invertase (StvacINV1) (β-fructofuranosidase; EC 3.2.1.26) has been confirmed to play an important role in cold-induced sweetening of potato tubers. However, the transcriptional regulation mechanisms of StvacINV1 are largely unknown. In this study, the 5â²-flanking sequence of StvacINV1 was cloned and the cis-acting elements were predicted. Histochemical assay showed that the StvacINV1 promoter governed β-glucuronidase (GUS) expression in potato leaves, stems, roots and tubers. Quantitative analysis of GUS expression suggested that the activity of StvacINV1 promoter was suppressed by sucrose, glucose, fructose, and cold, while enhanced by indole-3-acetic acid (IAA), and gibberellic acid (GA3). Further deletion analysis clarified that the promoter regions from â118 to â551, â551 to â1021, and â1021 to â1521 were required for responding to sucrose/glucose, GA3, and IAA, respectively. These findings provide essential information regarding transcriptional regulation mechanisms of StvacINV1.
Keywords
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Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Yongbin Ou, Botao Song, Xun Liu, Conghua Xie, Meng Li, Yuan Lin, Huiling Zhang, Jun Liu,