| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2014888 | Plant Physiology and Biochemistry | 2013 | 8 Pages |
Metallothioneins (MTs) play a major role in metal homeostasis and/or detoxification in plants. In this study, a novel gene, pCeMT, was isolated from Colocasia esculenta and characterized. Our results indicate that Escherichia coli cells expressing pCeMT exhibited enhanced Cd, Cu, and Zn tolerance and accumulation compared with control cells. Furthermore, pCeMT-overexpressing tobacco seedlings displayed better growth under Cd, Cu, and Zn stresses and accumulated more Cd and Zn compared with the wild type. Interestingly, transgenic tobacco displayed markedly decreased hydrogen peroxide (H2O2) and lipid peroxidation levels under Cd, Cu, and Zn treatments. These results suggest that pCeMT could play an important role in the protection of plant cells from oxidative stress by reactive oxygen species (ROS) scavenging and in the detoxification of free metals by metal binding, leading to improved plant metal tolerance.
► pCeMT was isolated from Colocasia esculenta. ► Overexpression of pCeMT enhanced Cd, Cu, and Zn tolerance in E. coli and tobacco. ► pCeMT affected Cd, Cu, and Zn accumulation in E. coli and tobacco. ► pCeMT prevented production of H2O2 and lipid peroxidation under heavy metal stress.
