| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2014948 | Plant Physiology and Biochemistry | 2013 | 12 Pages |
A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop “Moncada” [Clementine ‘Oroval’ (Citrus clementina Hort ex Tanaka) x ‘Kara’ mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS–MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction.
► A proteomic study was did to know molecular mechanisms of Citrus alternate bearing. ► The variety studied was “Moncada” mandarin, in the period of flowering induction. ► We researched protein expression differences between on-crop and off-crop leaves. ► The primary metabolism seemed more active in off-crop trees than in on-crop trees. ► Proteins up-expressed in on-crop leaves were more related to oxidoreductase activity.
