| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2014949 | Plant Physiology and Biochemistry | 2013 | 4 Pages |
The use of cyclic oligosaccharides like cyclodextrins (CDs), alone or combined with methyl jasmonate (MJ), as elicitors has proved very effective in stimulating the production of trans-resveratrol (trans-R) in Vitis vinifera suspension-cultured cells (SCC). Since elicitors can be used to increase trans-R production, understanding the molecular mechanisms involved would improve the management of grapevine cells as factories of this compound. The results obtained in this study provide evidence for a role of Ca2+ in mediating elicitor-induced trans-R production in grapevine SCC. The Ca2+ elevation was promoted by an uptake of Ca2+ from the extracellular medium, and by Ca2+ mobilization from intracellular organelles. Moreover, protein phosphorylation/dephosphorylation events seem to be involved in the signal transduction pathways triggered by CDs separately or in combination with MJ since trans-R production is dependent on both, the phosphorylation status of several proteins through mitogen-activated kinase pathway and the activity of tyrosine phosphatases. Our results also suggest that H2O2 and NO participated in the production of trans-R triggered by both elicitors in grapevine SCC. Finally, a fast alkalinization of the extracellular medium is induced in the presence of CDs and/or MJ.
► Ca2+ is essential for the activation of the biosynthesis of resveratrol. ► Phosphorylation/desphosphorylation events are essentials in resveratrol production. ► NO and H2O2 are involved in the signal transduction pathways induced by elicitors. ► Elicitors induce an alkalinization of the extracellular medium.
