Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2015688 | Plant Physiology and Biochemistry | 2016 | 10 Pages |
Abstract
Plant vascular patterning is complex. However, the detailed molecular mechanism of vascular patterning is still unknown. In this study, FBXL, an Arabidopsis F-box motif gene, was isolated by using 3Ⲡrapid amplification of cDNA ends (RACE) technique. The gene contained a coding sequence of 1407 nucleotides coding 468 amino acid residues. Amino acid sequence analysis revealed that the gene encoded a protein harboring an F-box motif at the N terminus, an LRRs motif in the middle, and an FBD motif at the C terminus. FBXL promoter-β-glucuronidase (GUS) and 35S promoter-FBXL vectors were constructed and transformed into Arabidopsis thaliana to understand the function of the FBXL gene. GUS expression analysis indicated that FBXL was specifically expressed in the vascular tissues of the root, stem, leaf, and inflorescence. FBXL overexpression in Arabidopsis displayed an abnormal venation pattern in cotyledons. Furthermore, FBXL expression was not induced by exogenous auxin and its transcript accumulation did not overlap with the distribution of endogenous auxin. These results suggested that FBXL may be involved in cotyledon vein patterning via auxin-independent pathway.
Keywords
5-Bromo-4-chloro-3-indolyl-β-D-glucuronideCAND1AUXIN RESISTANT1HVEAXR1SCFX-GlucGUSRT-PCRSFCqPCRLRRs2,4-D2,4-dichlorophenoxyacetic acidMurashige and Skoogβ-glucuronidaseArabidopsis thalianaOverexpressionrapid amplification of cDNA endsLeucine-rich repeatsDAGdays after germinationRacepolymerase chain reactionquantitative real-time polymerase chain reactionreverse-transcription polymerase chain reactionPCRPin1kinetin
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Authors
Xianghuan Cui, Xiaofeng Xu, Yangyang He, Xiling Du, Jian Zhu,