Leaf malate and succinate accumulation are out of phase throughout the development of the CAM plant Ananas comosus

•Organic acid in Ananas comosus are dependent on season and plant development.•Succinate was shown to have both diurnal and seasonal oscillations.•Malate and succinate are out of phase in A. comosus.

In plants with Crassulacean Acid Metabolism (CAM), organic acids, mainly malate are crucial intermediates for carbon fixation. In this research we studied the circadian oscillations of three organic anions (malate, citrate, and succinate) in Ananas comosus, assessing the effect of season and plant development stage. Seasonal and plant development dependencies were observed. The circadian oscillations of malate and citrate were typical of CAM pathways reported in the literature. Citrate content was quite stable (25–30 μmol g−1 FW) along the day, with a seasonal effect. Succinate was shown to have both diurnal and seasonal oscillations and also a correlation with malate, since it accumulated during the afternoon when malate content was normally at a minimum, suggesting a possible mechanistic effect between both anions in CAM and/or respiratory metabolisms.