| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2016048 | Plant Physiology and Biochemistry | 2013 | 7 Pages |
Abstract
⺠Purification of a novel PdKI-4 Kunitz inhibitor from Pithecellobium dumosum seeds with activity against serine proteinases. ⺠PdKI-4 also showed in vitro inhibitory activity toward Coleopterans, Lepidopterans and Dipterans digestive enzymes. ⺠Kinetic studies demonstrated that PdKI-4 is a competitive inhibitor.
Keywords
N-α-p-tosyl-l-lysine chloromethyl ketonen-α-benzoyl-dl-arginine-p-nitroanilideBANASBTIN-tosyl-l-phenylalanine chloromethyl ketoneTLCKBAPNATPCKPMSFTFATCAACNDMSOβ-mercaptoethanolβ-MeAcetonitrileTrifluoroacetic acidtrichloroacetic acidDimethylsulfoxidePhenylmethanesulfonyl fluorideinhibition constantTrypsin inhibitorsoybean Kunitz trypsin inhibitorhigh performance liquid chromatographyHPLCKunitz
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Agricultural and Biological Sciences
Plant Science
Authors
Fabiola P.S. Rufino, Vanessa M.A. Pedroso, Jonalson N. Araujo, Anderson F.J. França, Luciana M.A. Rabêlo, Ludovico Migliolo, Sumika Kiyota, Elizeu A. Santos, Octavio L. Franco, Adeliana S. Oliveira,