| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2016161 | Plant Physiology and Biochemistry | 2012 | 6 Pages |
We analyzed lipid kinase and lipid phosphatase activities and determined endogenous phytohormone levels by liquid chromatography–tandem mass spectrometry in root and coleoptile tissues following germination of barley (Hordeum vulgare) seeds. The enzymes showing highest activity in aleurone cells were diacylglycerol kinase (DAG-k, EC 2.7.1.107) and phosphatidate kinase (PA-k). The ratio of gibberellins (GAs) to abscisic acid (ABA) was 2-fold higher in aleurone than in coleoptile or root tissues. In coleoptiles, phosphatidylinositol 4-kinase (PI4-k, EC 2.7.1.67) showed the highest enzyme activity, and jasmonic acid (JA) level was higher than in aleurone. In roots, activities of PI4-k, DAG-k, and PA-k were similar, and salicylic acid (SA) showed the highest concentration. In the assays to evaluate the hydrolysis of DGPP (diacylglycerol pyrophosphate) and PA (phosphatidic acid) we observed that PA hydrolysis by LPPs (lipid phosphate phosphatases) was not modified; however, the diacylglycerol pyrophosphate phosphatase (DGPPase) was strikingly higher in coleoptile and root tissues than to aleurone. Relevance of these findings in terms of signaling responses and seedling growth is discussed.
► Lipid kinase/phosphatase and endogenous phytohormone were determinated in barley tissues. ► DAG-k and PA-k showed highest activity, GAs/ABA was 2-fold higher in aleurone. ► In coleoptile, PI4-k showed the highest enzyme activity, and JA level showed the greatest increase. ► In root, activities of PI4-k, DAG-k, and PA-k were similar, SA showed the highest concentration. ► DGPPase was strikingly increased in coleoptile and root compared to aleurone.
