| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2016492 | Plant Physiology and Biochemistry | 2011 | 5 Pages |
Lipoxygenase (linoleate:oxygen oxidoreductase; EC 1.13.11.12; LOX) catalyzes oxygenation of polyenoic fatty acids, which precedes the degradation of storage lipids during seed germination in sunflower. In the present work, it has been confirmed that 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) produces fluorescence in presence of lipid hydroperoxides (LOX reaction products). This work provides new information on spatial localization of transiently enhanced LOX activity in protoplasts from 5 d old seedling cotyledons of sunflower (Helianthus annuus L. cv. Morden) by exploiting H2DCFDA as a probe for fluorescence detection from LOX activity sites. Use of LOX inhibitors [nordihydroguaiaretic acid (NDGA) and propyl gallate (PG)] confirms oil bodies as LOX activity sites. Oil body surface has been shown to possess LOX activity in 5 d old seedling cotyledons.
Research highlights► LOX localization by fluorescence microscopy and CLSM. ► H2DCFDA oxidation by LOX. ► Oxidation product (2′,7′-dichlorofluorescein) is fluorescent. ► 2′,7′-dichlorofluorescein formation inhibited by LOX inhibitors. ► Enhanced LOX activity detected in seedling protoplasts and oil bodies.
