Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2016932 | Plant Science | 2016 | 11 Pages |
Abstract
An ErbB-3-binding protein gene AcEBP1, also known as proliferation-associated 2G4 gene (PA2G4s) belonging to the M24 superfamily, was obtained from the saltbush Atriplex canescens. Subcellular localization imaging showed the fusion protein AcEBP1-eGFP was located in the nucleus of epidermal cells in Nicotiana benthamiana. The AcEBP1 gene expression levels were up-regulated under salt, osmotic stress, and hormones treatment as revealed by qRT-PCR. Overexpression of AcEBP1 in Arabidopsis demonstrated that AcEBP1 was involved in root cell growth and stress responses (NaCl, osmotic stress, ABA, low temperature, and drought). These phenotypic data were correlated with the expression patterns of stress responsive genes and PR genes. The AcEBP1 transgenic Arabidopsis plants also displayed increased sensitivity under low temperature and evaluated resistance to drought stress. Together, these results demonstrate that AcEBP1 negatively affects cell growth and is a regulator under stress conditions.
Keywords
ABAPEG6000ChlAtriplex canescensPKCNBTeGFPcDNAComplementary DNAEF1αMurashige and SkoognMEROSnitroblue tetrazoliumArabidopsisEthyleneAbiotic stressabscisic acidoverexpressingChlorophyllSODSuperoxide dismutaseelongation factor 1-alphaMethyl jasmonateWater contentsMeJAwild-typepolymerase chain reactionPCRfresh weightdry weightpopPeroxidaseenhanced green fluorescent proteinProtein kinase CPolyethylene glycol 6000Vector controlReactive oxygen species
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Authors
Jingtao Li, Gang Yu, Xinhua Sun, Xianghui Zhang, Jinliang Liu, Hongyu Pan,