Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2016934 | Plant Science | 2016 | 14 Pages |
Abstract
Ubiquitination plays an important role in regulating plant's development and adaptability to abiotic stress. To investigate the possible functions of a wheat monoubiquitin gene Ta-Ub2 in abiotic stress in monocot and compare it with that in dicot, we generated transgenic Brachypodium plants overexpressing Ta-Ub2 under the control of CaMV35s and stress-inducible RD29A promoters. The constitutive expression of Ta-Ub2 displayed slight growth inhibition in the growth of transgenic Brachypodium distachyon under the control conditions. However, this inhibition was minimized by expression of Ta-Ub2 under the control of stress-inducible RD29A promoter. Compared with WT, the transgenic plants preserved more water and showed higher enzymatic antioxidants under drought stress, which might be related to the change in the expression of some antioxidant genes. The expression of C-repeat binding factors transcription factor genes in the transgenic B. distachyon lines were upregulated under water stress. Salt and cold tolerances of transgenic B. distachyon were also improved. Although the phenotypic changes in the transgenic plants were different, overexpression of Ta-Ub2 improved the abiotic stress tolerance in both dicot and monocot plants. The improvement in Ta-Ub2 transgenic plants in abiotic stress tolerance might be, at least partly, through regulating the gene expression and increasing the enzymatic antioxidants.
Keywords
Brachypodium distachyonPODCATCBFRWCAPXSWCROSEnzymatic antioxidantsAbiotic stress toleranceGrowth and developmentSODSuperoxide dismutaseC-repeat binding factorTranscription factorsSoil water contentRelative water contentwild typePeroxidaseascorbate peroxidasepolyethylene glycolPEGCatalaseReactive oxygen speciesUPSUbiquitin
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Hanhan Kang, Meng Zhang, Shumei Zhou, Qifang Guo, Fengjuan Chen, Jiajie Wu, Wei Wang,