Crystalline and amorphous cellulose in the secondary walls of Arabidopsis

In the cell walls of higher plants, cellulose chains are present in crystalline microfibril, with an amorphous part at the surface, or present as amorphous material. To assess the distribution and relative occurrence of the two forms of cellulose in the inflorescence stem of Arabidopsis, we used two carbohydrate-binding modules, CBM3a and CBM28, specific for crystalline and amorphous cellulose, respectively, with immunogold detection in TEM. The binding of the two CBMs displayed specific patterns suggesting that the synthesis of cellulose leads to variable nanodomains of cellulose structures according to cell type. In developing cell walls, only CBM3a bound significantly to the incipient primary walls, indicating that at the onset of its deposition cellulose is in a crystalline structure. As the secondary wall develops, the labeling with both CBMs becomes more intense. The variation of the labeling pattern by CBM3a between transverse and longitudinal sections appeared related to microfibril orientation and differed between fibers and vessels. Although the two CBMs do not allow the description of the complete status of cellulose microstructures, they revealed the dynamics of the deposition of crystalline and amorphous forms of cellulose during wall formation and between cell types adapting cellulose microstructures to the cell function.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Two specific CBMs were used to depict in planta crystalline and amorphous celluloses distribution in Arabidopsis. ► Crystalline and amorphous structures are differentially distributed between cell types and within wall sub-layers. ► Microfibril orientation influences the patterns of labeling. ► Crystalline forms of cellulose are absent at incipient stages of the forming wall. ► Cellulose microstructure is adapted to cell function.