Oxidative post translational modifications of proteins related to cell cycle are involved in cadmium toxicity in wheat seedlings

Abiotic stress is greatly associated with plant growth inhibition and redox cell imbalance. In the present work, we have investigated in which way oxidative posttranslational modifications (PTM) of proteins related to cell cycle may be implicated in post-germinative root growth reduction caused by cadmium, by methyl viologen (MV) and by hydrogen peroxide (H2O2) in wheat seedlings. Although cadmium is considered a redox inactive metal, reactive oxygen species were detected in the apex root of metal-treated seedlings. Oxidative stress hastened cells displacement from the cell division zone to elongation/differentiation zone, resulting in a shortened meristem. The number of cells in the proliferation zone was lower after MV, H2O2 and 10 μM Cd2+ treatments compared to control. All treatments increased protein carbonylation. Although no modification in total Ub-conjugated proteins was detected, oxidative treatments reduced cyclin D and CDKA protein ubiquitination, concomitantly with a decrease in expression of cyclin D/CDKA/Rb/E2F-regulated genes. We postulate that ROS and oxidative PTM could be part of a general mechanism, specifically affecting G1/S transition and progression through S phase. This would rapidly block cell cycle progression and would allow the cellular defence system to be activated.

Graphical abstractAlthough cadmium is considered a redox inactive metal, reactive oxygen species were detected in the apex root of metal-treated seedlings. Oxidative stress hastened cells displacement from the cell division zone to elongation/differentiation zone, resulting in a shortened meristem.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Abiotic stress is associated with plant growth inhibition and redox cell imbalance. ► Cell redox status is a key point in control of cell cycle progression under Cd stress. ► Oxidative PTM of proteins related to cell cycle may be involved in growth reduction. ► ROS and PTM could be part affecting G1/S transition and progression through S phase.