| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2017462 | Plant Science | 2012 | 15 Pages |
Cultivars of rice (Oryza sativa L.), especially the large-spikelet-type, often fail to achieve the high yield potential due to poor grain-filling of their inferior (late-flowering) spikelets. The superior (early-flowering) spikelets normally contain more abscisic acid (ABA) than the inferior spikelets. It was speculated that ABA might play a pivotal role in the grain-filling of inferior spikelets. To understand the molecular regulation involved in this process, we employed the 2-D gel-based comparative proteomic and phosphoproteomic analyses to search for differentially expressed proteins in the inferior spikelets under exogenous ABA treatment. A total of 111 significantly differential proteins and 31 phosphoproteins were found in the inferior spikelets after treatment. Among them, 100 proteins and 23 phosphoproteins were identified by using MALDI-TOF/TOF MS. In addition, the gene expression patterns of the inferior spikelets were confirmed with RT-PCR. These differentially expressed proteins are active in defense response, carbohydrate, protein, amino acid, energy and secondary metabolisms, as well as cell development and photosynthesis. The results suggest that the grain-filling of rice inferior spikelets is regulated by ABA through some proteins and phosphoproteins participating in carbon, nitrogen and energy metabolisms.
► Proteomic and phosphoproteomic assay were conducted on the inferior spikelets of rice with the 20 × 10−6 M ABA and control treatments. ► A total of 111 proteins and 31 phosphoproteins were found to differ significantly with the exotgroen ABA treatment. ► The gene expression patterns of the proteins were confirmed with RT-PCR. ► ABA-response proteins and phosphoproteins are likely involved in the grain filling of inferior spikelets.
