Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2018076 | Plant Science | 2009 | 8 Pages |
Abstract
The identification and characterization of promoters, cis-elements and transcription factors are critical for studying gene expression during growth and development in any organism. Use of specific promoters is an absolute requirement for the expression of foreign genes in plants in a developmentally, spatially and/or temporally regulated manner. The YY2 cDNA has been shown to be expressed specifically in the tapetum cells of rice anther. In this study, a 968Â bp upstream regulatory sequence (GenBank accession no. FJ957881) of the gene was isolated using genome walking method. The isolated fragment was shown to drive anther-specific expression of GUS reporter gene in rice as well as in the dicot plant Arabidopsis thaliana. Deletion analysis revealed that a â¼400Â bp 5â² regulatory region is sufficient to confer anther-specific expression of GUS gene.
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Agricultural and Biological Sciences
Plant Science
Authors
Boney Kuriakose, V. Arun, Samuel S. Gnanamanickam, George Thomas,