Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2018542 | Plant Science | 2007 | 11 Pages |
Abstract
The key to understanding the molecular mechanism of the defense response triggered by recognition of pathogen- or microbe-associated molecular patterns (PAMPs or MAMPs) is the identification and comparison of a set of early response genes to different microbial elicitors. We performed comprehensive and detailed monitoring of gene expression over time after application of two different microbial elicitors, PiE (an elicitor from the cell walls of an oomycete, Phytophthora infestans) and TvX (a xylanase from a fungus, Trichoderma viride), in tobacco cultured cells using the suppression subtractive hybridization and cDNA macroarray techniques. We identified various kinds of genes that are up- or down-regulated at the early stages of response to the elicitors. The majority of up-regulated genes are predicted to have a role in the defense response as signaling components and transcription factors as well as the metabolism involved in the production of secondary signaling molecules and anti-microbial compounds. The overall results revealed that there is no substantial difference in the expression profiles between cells treated with two different microbial elicitors, at least during the early phase of the defense response.
Keywords
CNGCOPPPcDNA macroarrayCDPKSSHelicitorsPAMPsGSTmicrobe-associated molecular patternspathogen-associated molecular patternsTobaccoMAMPSOxidative pentose phosphate pathwaySuppression subtractive hybridizationpolymerase chain reactionPCRPiemitogen-activated protein kinaseCalcium-dependent protein kinasecyclic nucleotide-gated ion channelMAP kinaseglutathione S-transferase
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Kaoru Suzuki, Akira Yano, Takumi Nishiuchi, Toshitsugu Nakano, Hiroaki Kodama, Kazuo Yamaguchi, Hideaki Shinshi,