Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2024342 | Soil Biology and Biochemistry | 2016 | 4 Pages |
Abstract
DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10Â g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high-throughput DNA metabarcoding studies of soil biodiversity.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Soil Science
Authors
Lucie Zinger, Jérôme Chave, Eric Coissac, Amaia Iribar, Eliane Louisanna, Sophie Manzi, Vincent Schilling, Heidy Schimann, Guilhem Sommeria-Klein, Pierre Taberlet,