Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2025036 | Soil Biology and Biochemistry | 2011 | 11 Pages |
Phaseolus vulgaris is a legume extensively cultivated in Spain, León province being the most important producer. This province produces selected varieties of common bean highly appreciated by their quality that warrants a Protected Geographic Indication (PGI). In this work we analysed the rhizobia present in nodules of the variety “Riñón” in several soils from León province in order to select native rhizobial strains to be used as biofertilizers. The analysis of rrs and housekeeping genes of these strains showed that they belong to two phylogenetic groups within Rhizobium leguminosarum (I and II). Although the group II strains were most abundant in nodules, very effective strains were also found in group I. Strains LCS0306 from group I and LBM1123 from group II were the best nitrogen fixers among all strains isolated and were selected for field experiments. The field research showed that the biofertilization of common bean with native and selected rhizobial strains can completely replace the fertilization with chemical N fertilizers. The biofertiliser designed in such way, was valid for the whole agroecological area, regardless the specific properties of each soil and microclimatic conditions. This conclusion can be generalised as a strategy for the development of biofertilisers in different agroecological conditions worldwide.
► First work about distribution of two different R. leguminosarum genotypes in common beans nodules in Northern Spanish soils. ► Selection of native strains representative of these genotypes by effectiveness in axenic conditions following field trial. ► Design of biofertilizers based in native strains useful for the whole ecogeographical production area, regardless the soil. ► Evidence that selected native strains can completely replace chemical N fertilization.