Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2027214 | Soil Biology and Biochemistry | 2006 | 6 Pages |
Abstract
To achieve stable expression of the heterologous and reporter genes in the Bradyrhizobium genome, we constructed suicide plasmids capable of site-directed genomic integration of the gusA, gfp and nifA genes by homologous recombination into non-essential repeated sequences (RS-α), isolated from B. japonicum strain CPAC7 (SEMIA5080). In this report, we describe the strategies to construct the vectors and their use to obtain mutants with site-specific insertions.
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Authors
Humberto J.O. Ramos, Emanuel M. Souza, Juliana R.L. Soares-Ramos, Fábio O. Pedrosa,