Developmental Priming of Stomatal Sensitivity to Abscisic Acid by Leaf Microclimate

•In Arabidopsis, transpiration decreases during leaf development•Stomata close while gradually acquiring their sensitivity to abscisic acid (ABA)•Rosette architecture induces a gradient in atmospheric relative humidity (RH)•Gradual exposure to low RH triggers the acquisition of stomatal sensitivity to ABA

SummaryPlant water loss and CO2 uptake are controlled by valve-like structures on the leaf surface known as stomata. Stomatal aperture is regulated by hormonal and environmental signals. We show here that stomatal sensitivity to the drought hormone abscisic acid (ABA) is acquired during leaf development by exposure to an increasingly dryer atmosphere in the rosette plant Arabidopsis. Young leaves, which develop in the center of the rosette, do not close in response to ABA. As the leaves increase in size, they are naturally exposed to increasingly dry air as a consequence of the spatial arrangement of the leaves, and this triggers the acquisition of ABA sensitivity. Interestingly, stomatal ABA sensitivity in young leaves is rapidly restored upon water stress. These findings shed new light on how plant architecture and stomatal physiology have coevolved to optimize carbon gain against water loss in stressing environments.

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