Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2047434 | FEBS Letters | 2015 | 7 Pages |
Abstract
We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-l-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile ÎamiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, ÎamiC mutant cells were unable to establish symbiotic association. But, ÎamiC mutant complemented with amiC gene restored in vivo symbiotic association. ÎamiC mutant cultured in minimal medium restored their motility with single-celled morphology. When ÎamiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Jun Beom Lee, Jin Hee Byeon, Ho Am Jang, Jiyeun Kate Kim, Jin Wook Yoo, Yoshitomo Kikuchi, Bok Luel Lee,