| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2047453 | FEBS Letters | 2015 | 7 Pages |
Abstract
CYP106A1 from Bacillus megaterium DSM319 was recently shown to catalyze steroid and terpene hydroxylations. Besides producing hydroxylated steroid metabolites at positions 6β, 7β, 9α and 15β, the enzyme displayed previously unknown 11-oxidase activity towards 11β-hydroxysteroids. Novel examples for 11-oxidation were identified and confirmed by 1H and 13C NMR for prednisolone, dexamethasone and 11β-hydroxyandrostenedione. However, only 11β-hydroxyandrostenedione formed a single 11-keto product. The latter reaction was chosen to investigate the kinetic solvent isotope effect on the steady-state turnover of the CYP106A1-mediated 11-oxidation. Our results reveal a large inverse kinetic isotope effect (â¼0.44) suggesting the involvement of the ferric peroxoanion as a reactive intermediate.
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Authors
Flora Marta Kiss, Yogan Khatri, Josef Zapp, Rita Bernhardt,