| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2047635 | FEBS Letters | 2015 | 5 Pages |
•VAMP-2 was identified as a target of miR-206.•miR-206 decreased the expression of VAMP-2 protein.•miR-206 reduced lung surfactant secretion in alveolar type II cells.
Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3′-untranslational region (3′-UTR) luciferase assay. Mutations in the predicated miR-206 binding sites reduced the binding of miR-206 to the 3′-UTR of VAMP-2. miR-206 decreased the expression of VAMP-2 protein and decreased the lung surfactant secretion in alveolar type II cells. In conclusion, miR-206 regulates lung surfactant secretion by limiting the availability of VAMP-2 protein.
