MiR-483–5p suppresses the proliferation of glioma cells via directly targeting ERK1

MicroRNAs (miRNAs) exhibit tumor-specific expression signatures and play crucial roles in tumorigenesis by targeting oncogenes. Here, through analyzing the miRNA-array profiles of human glioblastoma tissues and the adjacent normal brain tissues, we found miR-483–5p was significantly down-regulated in gliomas, which was confirmed in both human glioma specimens and cell lines. The overexpression of miR-483–5p suppressed glioma cell proliferation and induced a G0/G1 arrest. In contrast, miR-483–5p inhibition promoted cell proliferation. Furthermore, by a dual-luciferase reporter assay and expression analysis, we identified extracellular signal-regulated kinase 1 (ERK1) as a direct target of miR-483–5p. ERK1 knockdown can block cell proliferation induced by miR-483–5p inhibition. Thus, our findings provide the first evidence that miR-483–5p can serve as a tumor suppressor in gliomas.

► We identified a novel down-regulated miRNA, miR-483–5p, in gliomas. ► We found that miR-483–5p inhibits glioma cell proliferation by inducing G0/G1 arrest. ► We verified that ERK1 is a direct target of miR-483–5p. ► We found that miR-483–5p inhibited glioma cell proliferation by targeting ERK1.