The Junctional Adhesion Molecule-B regulates JAM-C-dependent melanoma cell metastasis

Metastasis is a major clinical issue and results in poor prognosis for most cancers. The Junctional Adhesion Molecule-C (JAM-C) expressed by B16 melanoma and endothelial cells has been involved in metastasis of tumor cells through homophilic JAM-C/JAM-C trans-interactions. Here, we show that JAM-B expressed by endothelial cells contributes to murine B16 melanoma cells metastasis through its interaction with JAM-C on tumor cells. We further show that this adhesion molecular pair mediates melanoma cell adhesion to primary Lung Microvascular Endothelial Cells and that it is functional in vivo as demonstrated by the reduced metastasis of B16 cells in Jam-b deficient mice.

► JAM-B is highly expressed on Lung Microvascular Endothelial Cells. ► JAM-B/JAM-C interaction mediates melanoma cell adhesion to endothelial cells in vitro. ► JAM-B/JAM-C interaction contributes to B16F10 melanoma cells metastasis.