Article ID Journal Published Year Pages File Type
2048038 FEBS Letters 2013 7 Pages PDF
Abstract

•Y-box binding protein 1 (YB1) binds directly to GC boxes in target gene promoters.•All-trans-retinoic acid (ATRA) influences YB1 recruitment to GC boxes in VSMCs. YB1 binds to GC boxes via amino acids 125–220.•YB1 functions as a transcription activator or suppressor depending on gene context.•The YB1 C-terminal tail domain suppresses VSMC proliferation.

Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a key event in atherosclerosis and restenosis. In this paper, we report that Y-box binding protein 1 (YB1) functions as a phenotypic regulator in VSMC proliferation-differentiation switching through targeting GC box-dependent genes. Oligo pull-down assays demonstrated that YB1 binds directly to GC boxes via amino acids 125–220. YB1 C-terminal tail domain (CTD, amino acids 125–324) regulates GC box-dependent target gene transcription and suppresses VSMC proliferation. These findings provide a novel insight into the regulation of GC box-related genes by YB1, and provide a new understanding of VSMC proliferation regulation.

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