The murine Gcap14 gene encodes a novel microtubule binding and bundling protein

Microtubules form flexible fibers, which are utilized in cell proliferation and differentiation. Although the flexibility of microtubules was shown to be regulated by various microtubule-associated proteins, this regulation is still far from complete understanding. Here, we report a new potential regulator of microtubules in mammals. Gcap14 colocalizes with microtubules in mammalian cells transfected with Gcap14 expression vector. Association of Gcap14 with microtubules was confirmed by biochemical subcellular fractionation. Recombinant Gcap14 protein cosedimented with pure microtubules, indicating a direct binding between the two. Furthermore, recombinant Gcap14 was shown to have the ability of inducing microtubule bundling in vitro.Structured summary of protein interactionsGcap14physically interacts with Gcap14 by anti tag coimmunoprecipitation (View Interaction: 1, 2)

► Gcap14 is a newly found microtubule-associated protein in mammals. ► Gcap14 is shown to bind to microtubules directly. ► Gcap14 induces bundling of microtubules in vitro.