Article ID Journal Published Year Pages File Type
2048291 FEBS Letters 2012 6 Pages PDF
Abstract

Gonadotropin-releasing hormone (GnRH) regulates the synthesis and secretion of follicle-stimulating hormone (FSH) by stimulating the transcription of Fshβ gene. Our iTRAQ quantitative proteomics result showed that the abundance of α-actinin4 (ACTN4) increased in the nuclei of LβT2 cells upon GnRH induction. Using RNA interference, reverse transcription and real-time PCR, luciferase and transient transfection assays, we proved that ACTN4 is involved in the regulation of mouse Fshβ gene (mFshβ) transcription and its C-terminal calmodulin (CaM)-like domain is crucial for this process. Our study suggests that ACTN4 nuclear translocation mediates GnRH stimulation of mFshβ gene transcription.

► GnRH induces ACTN4 nuclear translocation. ► ACTN4 is involved in the regulation of mFshβ gene transcription. ► C-terminal CaM-like domain of ACTN4 is crucial for the above regulation. ► Both Ca2+ signaling pathway and MAPK pathway play roles in GnRH gene regulation.

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