Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2048322 | FEBS Letters | 2012 | 5 Pages |
Abstract
A previously uncharacterized Arabidopsis lecithin:cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine > phosphatidic acid > phosphatidylethanolamine > phosphatidylglycerol > phosphatidylserine and has an acyl chain specificity for oleoyl > linoleoyl > ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques.
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Authors
Guanqun Chen, Michael S. Greer, Ida Lager, Jenny Lindberg Yilmaz, Elzbieta Mietkiewska, Anders S. Carlsson, Sten Stymne, Randall J. Weselake,